Journal: BMC Neuroscience

Article Title: Lipopolysaccharide-induced inflammation attenuates taste progenitor cell proliferation and shortens the life span of taste bud cells

doi: 10.1186/1471-2202-11-72

Figure Lengend Snippet: LPS-induced inflammation downregulates the expression of cyclin B2 and E2F1 in the circumvallate epithelium . A, Scatter plot summarizing gene expression analysis using the Mouse Cell Cycle RT 2 Profiler PCR Array. The expression of 84 genes involved in cell cycle regulation was analyzed by real-time RT-PCR in circumvallate and foliate epithelial samples prepared 24 h after PBS (x-axis) or LPS (y-axis) injection. Each circle represents a gene in the PCR array. The middle diagonal gray line indicates fold change of 1 (no change of gene expression between PBS and LPS groups). Circles above this gray line indicate increased gene expression, and circles below, decreased expression, in LPS samples versus PBS samples. The two outside gray lines indicate a fold change of 4. The five green circles represent genes that showed >4-fold decreased expression in LPS samples. B, C, Independent real-time RT-PCR expression analyses of cyclin B2 (B) and E2F1 (C) in circumvallate and foliate epithelia prepared 24 h after PBS or LPS injection. LPS significantly decreased the expression of both genes in circumvallate and foliate epithelia. Error bars indicate SEM. ** p < 0.005.

Article Snippet: Quantitative real-time PCR was set up using Power SYBR Green PCR Master Mix (Applied Biosystems) and PCR primer sets from the Mouse Cell Cycle RT 2 Profiler PCR Array (PAMM-020A, SABiosciences).

Techniques: Expressing, Quantitative RT-PCR, Injection